|
|
|
|
|
|
|
||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
The Journal of General Physiology, Vol 103, 605-623, Copyright © 1994 by The Rockefeller University Press
ARTICLES |
F Jaisser, P Jaunin, K Geering, BC Rossier and JD Horisberger
Institut de Pharmacologie et de Toxicologie, Lausanne, Switzerland.
To study the role of the Na,K-ATPase beta subunit in the ion transport activity, we have coexpressed the Bufo alpha 1 subunit (alpha 1) with three different isotypes of beta subunits, the Bufo Na,K-ATPase beta 1 (beta 1NaK) or beta 3 (beta 3NaK) subunit or the beta subunit of the rabbit gastric H,K-ATPase (beta HK), by cRNA injection in Xenopus oocyte. We studied the K+ activation kinetics by measuring the Na,K- pump current induced by external K+ under voltage clamp conditions. The endogenous oocyte Na,K-ATPase was selectively inhibited, taking advantage of the large difference in ouabain sensitivity between Xenopus and Bufo Na,K pumps. The K+ half-activation constant (K1/2) was higher in the alpha 1 beta 3NaK than in the alpha 1 beta 1NaK groups in the presence of external Na+, but there was no significant difference in the absence of external Na+. Association of alpha 1 and beta HK subunits produced active Na,K pumps with a much lower apparent affinity for K+ both in the presence and in the absence of external Na+. The voltage dependence of the K1/2 for external K+ was similar with the three beta subunits. Our results indicate that the beta subunit has a significant influence on the ion transport activity of the Na,K pump. The small structural differences between the beta 1NaK and beta 3NaK subunits results in a difference of the apparent affinity for K+ that is measurable only in the presence of external Na+, and thus appears not to be directly related to the K+ binding site. In contrast, association of an alpha 1 subunit with a beta HK subunit results in a Na,K pump in which the K+ binding or translocating mechanisms are altered since the apparent affinity for external K+ is affected even in the absence of external Na+.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Bibert, S. Roy, D. Schaer, J.-D. Horisberger, and K. Geering Phosphorylation of Phospholemman (FXYD1) by Protein Kinases A and C Modulates Distinct Na,K-ATPase Isozymes J. Biol. Chem., January 4, 2008; 283(1): 476 - 486. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Madan, K. Rose, and A. J. Watson Na/K-ATPase beta1 Subunit Expression Is Required for Blastocyst Formation and Normal Assembly of Trophectoderm Tight Junction-associated Proteins J. Biol. Chem., April 20, 2007; 282(16): 12127 - 12134. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Delprat, D. Schaer, S. Roy, J. Wang, J.-L. Puel, and K. Geering FXYD6 Is a Novel Regulator of Na,K-ATPase Expressed in the Inner Ear J. Biol. Chem., March 9, 2007; 282(10): 7450 - 7456. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Bibert, S. Roy, D. Schaer, E. Felley-Bosco, and K. Geering Structural and Functional Properties of Two Human FXYD3 (Mat-8) Isoforms J. Biol. Chem., December 22, 2006; 281(51): 39142 - 39151. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. E. Dempski, K. Hartung, T. Friedrich, and E. Bamberg Fluorometric Measurements of Intermolecular Distances between the {alpha}- and beta-Subunits of the Na+/K+-ATPase J. Biol. Chem., November 24, 2006; 281(47): 36338 - 36346. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. M. Charron, M. G. Blanchard, and J.-Y. Lapointe Intracellular Hypertonicity Is Responsible for Water Flux Associated with Na+/Glucose Cotransport Biophys. J., May 15, 2006; 90(10): 3546 - 3554. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Capendeguy, P. Chodanowski, O. Michielin, and J.-D. Horisberger Access of Extracellular Cations to their Binding Sites in Na,K-ATPase: Role of the Second Extracellular Loop of the {alpha} Subunit J. Gen. Physiol., February 27, 2006; 127(3): 341 - 352. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Li, G. Crambert, D. Thuillard, S. Roy, D. Schaer, and K. Geering Role of the Transmembrane Domain of FXYD7 in Structural and Functional Interactions with Na,K-ATPase J. Biol. Chem., December 30, 2005; 280(52): 42738 - 42743. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Lubarski, K. Pihakaski-Maunsbach, S. J. D. Karlish, A. B. Maunsbach, and H. Garty Interaction with the Na,K-ATPase and Tissue Distribution of FXYD5 (Related to Ion Channel) J. Biol. Chem., November 11, 2005; 280(45): 37717 - 37724. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Li, O. Capendeguy, K. Geering, and J.-D. Horisberger A third Na+-binding site in the sodium pump PNAS, September 6, 2005; 102(36): 12706 - 12711. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Capendeguy and J.-D. Horisberger The role of the third extracellular loop of the Na+,K+-ATPase {alpha} subunit in a luminal gating mechanism J. Physiol., May 15, 2005; 565(1): 207 - 218. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Crambert, C. Li, D. Claeys, and K. Geering FXYD3 (Mat-8), a New Regulator of Na,K-ATPase Mol. Biol. Cell, May 1, 2005; 16(5): 2363 - 2371. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. E. Dempski, T. Friedrich, and E. Bamberg The {beta} Subunit of the Na+/K+-ATPase Follows the Conformational State of the Holoenzyme J. Gen. Physiol., April 25, 2005; 125(5): 505 - 520. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Li, A. Grosdidier, G. Crambert, J.-D. Horisberger, O. Michielin, and K. Geering Structural and Functional Interaction Sites between Na,K-ATPase and FXYD Proteins J. Biol. Chem., September 10, 2004; 279(37): 38895 - 38902. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Crambert, C. Li, L. K. Swee, and K. Geering FXYD7, Mapping of Functional Sites Involved in Endoplasmic Reticulum Export, Association With and Regulation of Na,K-ATPase J. Biol. Chem., July 16, 2004; 279(29): 30888 - 30895. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-D. Horisberger, S. Kharoubi-Hess, S. Guennoun, and O. Michielin The Fourth Transmembrane Segment of the Na,K-ATPase {alpha} Subunit: A SYSTEMATIC MUTAGENESIS STUDY J. Biol. Chem., July 9, 2004; 279(28): 29542 - 29550. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Zhao, N. B. Pestov, T. V. Korneenko, M. I. Shakhparonov, and N. N. Modyanov Accumulation of {beta}m, a structural member of X,K-ATPase {beta}-subunit family, in nuclear envelopes of perinatal myocytes Am J Physiol Cell Physiol, April 1, 2004; 286(4): C757 - C767. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Laughery, M. L. Todd, and J. H. Kaplan Mutational Analysis of {alpha}-{beta} Subunit Interactions in the Delivery of Na,K-ATPase Heterodimers to the Plasma Membrane J. Biol. Chem., September 12, 2003; 278(37): 34794 - 34803. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Burnay, G. Crambert, S. Kharoubi-Hess, K. Geering, and J.-D. Horisberger Electrogenicity of Na,K- and H,K-ATPase Activity and Presence of a Positively Charged Amino Acid in the Fifth Transmembrane Segment J. Biol. Chem., May 23, 2003; 278(21): 19237 - 19244. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Crambert, M. Fuzesi, H. Garty, S. Karlish, and K. Geering Phospholemman (FXYD1) associates with Na,K-ATPase and regulates its transport properties PNAS, August 20, 2002; 99(17): 11476 - 11481. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Crambert, J.-D. Horisberger, N. N. Modyanov, and K. Geering Human nongastric H+-K+-ATPase: transport properties of ATP1al1 assembled with different beta -subunits Am J Physiol Cell Physiol, July 1, 2002; 283(1): C305 - C314. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Burnay, G. Crambert, S. Kharoubi-Hess, K. Geering, and J.-D. Horisberger Bufo marinus bladder H-K-ATPase carries out electroneutral ion transport Am J Physiol Renal Physiol, November 1, 2001; 281(5): F869 - F874. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Crambert, U. Hasler, A. T. Beggah, C. Yu, N. N. Modyanov, J.-D. Horisberger, L. Lelievre, and K. Geering Transport and Pharmacological Properties of Nine Different Human Na,K-ATPase Isozymes J. Biol. Chem., January 21, 2000; 275(3): 1976 - 1986. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Shainskaya, A. Schneeberger, H.-J. Apell, and S. J. D. Karlish Entrance Port for Na+ and K+ Ions on Na+,K+-ATPase in the Cytoplasmic Loop between Trans-membrane Segments M6 and M7 of the alpha Subunit. PROXIMITY OF THE CYTOPLASMIC SEGMENT OF THE beta SUBUNIT J. Biol. Chem., January 21, 2000; 275(3): 2019 - 2028. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. K. Wetzel, E. Arystarkhova, and K. J. Sweadner Cellular and Subcellular Specification of Na,K-ATPase alpha and beta Isoforms in the Postnatal Development of Mouse Retina J. Neurosci., November 15, 1999; 19(22): 9878 - 9889. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. R. Burrow, O. Devuyst, X. Li, L. Gatti, and P. D. Wilson Expression of the beta 2-subunit and apical localization of Na+-K+-ATPase in metanephric kidney Am J Physiol Renal Physiol, September 1, 1999; 277(3): F391 - F403. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Asano, T. Kimura, S. Ueno, M. Kawamura, and N. Takeguchi Chimeric Domain Analysis of the Compatibility between H+,K+-ATPase and Na+,K+-ATPase beta -Subunits for the Functional Expression of Gastric H+,K+-ATPase J. Biol. Chem., August 6, 1999; 274(32): 22257 - 22265. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Jaisser and A. T. Beggah The nongastric H+-K+-ATPases: molecular and functional properties Am J Physiol Renal Physiol, June 1, 1999; 276(6): F812 - F824. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. B. Shimon, R. Goldshleger, and S. J. D. Karlish Specific Cu2+-catalyzed Oxidative Cleavage of Na,K-ATPase at the Extracellular Surface J. Biol. Chem., December 18, 1998; 273(51): 34190 - 34195. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Hasler, X. Wang, G. Crambert, P. Beguin, F. Jaisser, J.-D. Horisberger, and K. Geering Role of beta -Subunit Domains in the Assembly, Stable Expression, Intracellular Routing, and Functional Properties of Na,K-ATPase J. Biol. Chem., November 13, 1998; 273(46): 30826 - 30835. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Blanco and R. W. Mercer Isozymes of the Na-K-ATPase: heterogeneity in structure, diversity in function Am J Physiol Renal Physiol, November 1, 1998; 275(5): F633 - F650. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Beguin, U. Hasler, A. Beggah, J.-D. Horisberger, and K. Geering Membrane Integration of Na,K-ATPase alpha -Subunits and beta -Subunit Assembly J. Biol. Chem., September 18, 1998; 273(38): 24921 - 24931. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P.-X. Chen, P. M. Mathews, P. J. Good, B. C. Rossier, and K. Geering Unusual degradation of alpha -beta complexes in Xenopus oocytes by beta -subunits of Xenopus gastric H-K-ATPase Am J Physiol Cell Physiol, July 1, 1998; 275(1): C139 - C145. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Lambrecht, Z. Corbett, D. Bayle, S. J. D. Karlish, and G. Sachs Identification of the Site of Inhibition by Omeprazole of a alpha -beta Fusion Protein of the H,K-ATPase Using Site-directed Mutagenesis J. Biol. Chem., May 29, 1998; 273(22): 13719 - 13728. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Arystarkhova and K. J. Sweadner Tissue-specific Expression of the Na,K-ATPase beta 3 Subunit. THE PRESENCE OF beta 3 IN LUNG AND LIVER ADDRESSES THE PROBLEM OF THE MISSING SUBUNIT J. Biol. Chem., September 5, 1997; 272(36): 22405 - 22408. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Liu and A. Askari Evidence for the Existence of Two ATP-sensitive Rb+ Occlusion Pockets within the Transmembrane Domains of Na+/K+-ATPase J. Biol. Chem., May 30, 1997; 272(22): 14380 - 14386. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. E. Colonna, L. Huynh, and D. M. Fambrough Subunit Interactions in the Na,K-ATPase Explored with the Yeast Two-hybrid System J. Biol. Chem., May 9, 1997; 272(19): 12366 - 12372. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. T. Beggah, P. Jaunin, and K. Geering Role of Glycosylation and Disulfide Bond Formation in the beta Subunit in the Folding and Functional Expression of Na,K-ATPase J. Biol. Chem., April 11, 1997; 272(15): 10318 - 10326. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Codina, B. C. Kone, J. T. Delmas-Mata, and T. D. DuBose Jr. Functional Expression of the Colonic H+,K+-ATPase alpha -Subunit. PHARMACOLOGIC PROPERTIES AND ASSEMBLY WITH X+,K+-ATPase beta -SUBUNITS J. Biol. Chem., November 22, 1996; 271(47): 29759 - 29763. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. R. Simon, J. Fortune, A. Alexander, M. Iwahashi, R. Dahl, and E. Sutherland Increased Hepatic Na,K-ATPase Activity during Hepatic Regeneration Is Associated with Induction of the beta 1-Subunit and Expression on the Bile Canalicular Domain J. Biol. Chem., October 4, 1996; 271(40): 24967 - 24975. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Shainskaya and S. J. D. Karlish Chymotryptic Digestion of the Cytoplasmic Domain of the beta Subunit of Na/K-ATPase Alters Kinetics of Occlusion of Rb[IMAGE] Ions J. Biol. Chem., April 26, 1996; 271(17): 10309 - 10316. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Cougnon, G. Planelles, M. S. Crowson, G. E. Shull, B. C. Rossier, and F. Jaisser The Rat Distal Colon P-ATPase alpha Subunit Encodes a Ouabain-sensitive H[IMAGE],K[IMAGE]-ATPase J. Biol. Chem., March 29, 1996; 271(13): 7277 - 7280. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. G. Therien, N. B. Nestor, W. J. Ball, and R. Blostein Tissue-specific Versus Isoform-specific Differences in Cation Activation Kinetics of the Na,K-ATPase J. Biol. Chem., March 22, 1996; 271(12): 7104 - 7112. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Or, R. Goldshleger, and S. J. D. Karlish An Effect of Voltage on Binding of Na[IMAGE] at the Cytoplasmic Surface of the Na[IMAGE]-K[IMAGE] Pump J. Biol. Chem., February 2, 1996; 271(5): 2470 - 2477. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. A. Sarvazyan, N. N. Modyanov, and A. Askari Intersubunit and Intrasubunit Contact Regions of Na[IMAGE]/K[IMAGE]-ATPase Revealed by Controlled Proteolysis and Chemical Cross-linking J. Biol. Chem., November 3, 1995; 270(44): 26528 - 26532. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Hasler, P. J. Greasley, G. von Heijne, and K. Geering Determinants of Topogenesis and Glycosylation of Type II Membrane Proteins. ANALYSIS OF Na,K-ATPase beta 1 AND beta 3 SUBUNITS BY GLYCOSYLATION MAPPING J. Biol. Chem., September 8, 2000; 275(37): 29011 - 29022. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Hasler, G. Crambert, J.-D. Horisberger, and K. Geering Structural and Functional Features of the Transmembrane Domain of the Na,K-ATPase beta Subunit Revealed by Tryptophan Scanning J. Biol. Chem., May 4, 2001; 276(19): 16356 - 16364. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-D. Horisberger and S. Kharoubi-Hess Functional differences between alpha subunit isoforms of the rat Na,K-ATPase expressed in Xenopus oocytes J. Physiol., March 15, 2002; 539(3): 669 - 680. [Abstract] [Full Text] [PDF]
|
|
|
|
