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J. Gen. Physiol.,
Volume 111, Number 1, January 1, 1998 127-138
From the Institute of Pharmacology and Toxicology, University of Lausanne, CH-1005 Lausanne, Switzerland
We have investigated the effect of extracellular proteases on the amiloride-sensitive Na+ current
(INa) in Xenopus oocytes expressing the three subunits 
, 
, and 
of the rat or Xenopus epithelial Na+ channel
(ENaC). Low concentrations of trypsin (2 µg/ml) induced a large increase of INa within a few minutes, an effect
that was fully prevented by soybean trypsin inhibitor, but not by amiloride. A similar effect was observed with chymotrypsin, but not with kallikrein. The trypsin-induced increase of INa was observed with Xenopus and rat ENaC,
and was very large (~20-fold) with the channel obtained by coexpression of the subunit of Xenopus ENaC with the and subunits of rat ENaC. The effect of trypsin was selective for ENaC, as shown by the absence of effect on
the current due to expression of the K+ channel ROMK2. The effect of trypsin was not prevented by intracellular injection of EGTA nor by pretreatment with GTP-S, suggesting that this effect was not mediated by G proteins.
Measurement of the channel protein expression at the oocyte surface by antibody binding to a FLAG epitope
showed that the effect of trypsin was not accompanied by an increase in the channel protein density, indicating
that proteolysis modified the activity of the channel present at the oocyte surface rather than the cell surface expression. At the single channel level, in the cell-attached mode, more active channels were observed in the patch
when trypsin was present in the pipette, while no change in channel activity could be detected when trypsin was
added to the bath solution around the patch pipette. We conclude that extracellular proteases are able to increase the open probability of the epithelial sodium channel by an effect that does not occur through activation of a G
protein-coupled receptor, but rather through proteolysis of a protein that is either a constitutive part of the channel itself or closely associated with it.
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