The Journal of General Physiology
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The Journal of General Physiology, Vol 16, 615-623, Copyright © 1933 by The Rockefeller University Press


ARTICLE

CRYSTALLINE PEPSIN

V. ISOLATION OF CRYSTALLINE PEPSIN FROM BOVINE GASTRIC JUICE



John H. Northrop 1

1 From the Laboratories of The Rockefeller Institute for Medical Research, Princeton, N. J.

1. A method has been described for isolating a crystalline protein with high proteolytic activity from bovine gastric juice by means of precipitation with magnesium sulfate and fractionation of the precipitate with acetone and magnesium sulfate.

2. The crystalline protein obtained in this way has the same crystalline form, optical activity, and specific activity, as determined by a number of methods, as does the crystalline protein previously isolated from swine gastric mucosa.

3. The solubility of the two preparations, however, is additive so that they are different although very closely related proteins.

Accepted on January 10, 1933


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