The Journal of General Physiology
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The Journal of General Physiology, Vol 22, 281-292, Copyright © 1939 by The Rockefeller University Press

ARTICLE

THE INFLUENCE OF THE MOLECULAR WEIGHT OF ANTIGEN ON THE PROPORTION OF ANTIBODY TO ANTIGEN IN PRECIPITATES

II. A STATISTICAL EXAMINATION OF AVAILABLE DATA, INCLUDING SOME PREVIOUSLY UNPUBLISHED



William C. Boyd 1 and Sanford B. Hooker 1

1 From the Boston University School of Medicine and the Evans Memorial, Massachusetts Memorial Hospitals, Boston

A statistical examination of the available data on the ratio of antibody to antigen in specific precipitates made at or near the optimum shows a definite correlation between the ratio and the molecular weight of antigen (regression coefficient = –0.529 (±0.014)). The authors' assumption that at this point the antigen molecules are just about covered by a layer of antibody behaving as contiguous spherical ("Svedberg") units of weight 35,200 leads to predicted ratios that in general agree well with those found, though individual experimental determinations may deviate considerably.

Accepted on October 7, 1938


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