The Journal of General Physiology
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The Journal of General Physiology, Vol 25, 523-531, Copyright © 1942 by The Rockefeller University Press

ARTICLE

QUANTITATIVE CHEMICAL STUDIES ON HEMOLYSINS

I. THE ESTIMATION OF TOTAL ANTIBODY IN ANTISERA TO SHEEP ERYTHROCYTES AND STROMATA



Michael Heidelberger 1 and Henry P. Treffers 1

1 From the Department of Medicine, College of Physicians and Surgeons, Columbia University, and the Presbyterian Hospital, New York

1. Total antibody in hemolysins may be estimated from the nitrogen added to sheep stromata suspensions.

2. The method is applied to a number of hemolysins and a correlation, valid to within 20 per cent, established between hemolytic titer and total antibody.

3. When stromata combine with antibody in the presence of guinea pig complement they may take up at least 80 per cent of their weight of complement combining component(s).

Submitted on December 2, 1941


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