The Journal of General Physiology
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The Journal of General Physiology, Vol 34, 81-85, Copyright © 1950 by The Rockefeller University Press

ARTICLE

TISSUE CULTURE STUDIES

VI. THE EFFECT OF MEDIUM CONSTITUENTS ON NUCLEIC ACIDS AND UPTAKE OF P32



Wayne Hull 1 and Paul L. Kirk 1

1 From the Division of Biochemistry, University of California Medical School, Berkeley

The effect of horse serum alone, and of embryo extract alone, was compared with that of "complete medium" on the content and synthesis of ribo- and desoxyribonucleic acids and uptake of tracer P32 by chick heart cultures in vitro.

The factors mentioned are influenced by embryo extract in a manner similar to the effect in complete medium. Horse serum produced little synthesis of nucleic acids or uptake of tracer, giving only slightly more effect than Tyrode's solution alone.

Cutting the tissue into smaller pieces caused considerably greater synthetic effects, and retarded necrosis of the implant.

Submitted on July 13, 1950


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S. Rosenberg and P. L. Kirk
An Ultrafilterable Growth Factor for Tissue Culture
Science, May 22, 1953; 117(3047): 566 - 566.
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