The Journal of General Physiology
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The Journal of General Physiology, Vol 34, 225-229, Copyright © 1950 by The Rockefeller University Press

ARTICLE

PHAGE FORMATION IN STAPHYLOCOCCUS MUSCAE CULTURES

VII. PARTIAL PURIFICATION OF THE PROTEIN FACTOR NECESSARY FOR VIRUS SYNTHESIS



Winston H. Price 1

1 From the Laboratories of The Rockefeller Institute for Medical Research, Princeton, New Jersey

1. A substance is present in autolyzed pepsin solutions which stimulates the release of phage by some strains of S. muscae when added to Fildes' synthetic medium.

2. The substance is assayed by determining the quantity necessary to increase the phage yield to one-half the maximum value, using the one-step growth curve technique.

3. The substance has been concentrated and partially purified (500-fold) by heavy metal precipitation, butyl alcohol extraction, and absorption on norit.

4. No known amino acid or accessory growth substance tested could replace this substance.

Submitted on July 18, 1950


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