The Journal of General Physiology
Avanti Polar Lipids, Inc.
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text (PDF, 619K)
Right arrow Alert me when this article is cited
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JGP
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ajl, S. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ajl, S. J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
The Journal of General Physiology, Vol 34, 785-794, Copyright © 1951 by The Rockefeller University Press

ARTICLE

STUDIES ON THE MECHANISM OF ACETATE OXIDATION BY BACTERIA

V. EVIDENCE FOR THE PARTICIPATION OF FUMARATE, MALATE, AND OXALACETATE IN THE OXIDATION OF ACETIC ACID BY ESCHERICHIA COLI



Samuel J. Ajl 1

1 From the Department of Bacteriology and Immunology, Washington University School of Medicine, St. Louis

1. Simultaneous oxidation of C14-methyl-labeled acetate, and unlabeled malate or fumarate and alpha-ketoglutarate results in entrapment of labeled carbon in the C4-dicarboxylic acids, but not in alpha-ketoglutarate, although all substrates are utilized at comparable rates.

2. A large endogenous reduction of all C4-dicarboxylic acids (fumarate, oxalacetate, and malate) to succinate is observed under aerobic conditions, and when vigorous oxidation is proceeding. This effect occurs with both freshly harvested young (18 hour) cells and stored (2 week) cells.

3. This reduction can be considerably minimized under high oxygen tensions.

4. The quantitative concordance of these results with a Thunberg-Knoop cyclic mechanism for acetate oxidation is shown. Possible alternative C4 products formed prior to succinate are not completely excluded, but it appears that the cells can utilize the succinate condensation as a major pathway in acetate oxidation.

Submitted on January 12, 1951


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?




  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents