The Journal of General Physiology
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The Journal of General Physiology, Vol 35, 529-557, Copyright © 1952 by The Rockefeller University Press

ARTICLE

THE ISOLATION OF CELL NUCLEI IN NON-AQUEOUS MEDIA

V. Allfrey 1, H. Stern 1, A. E. Mirsky 1, and H. Saetren 1

1 From the Laboratories of The Rockefeller Institute for Medical Research

1. A modified Behrens procedure is described for the isolation of nuclei from avian erythrocytes and from the liver, kidney, thymus, pancreas, heart, and intestinal mucosa of the calf or horse.

2. The purity of these nuclei has been established by staining reactions, enzyme studies, and immunological tests for serum proteins.

3. Evidence is presented to show that a transport of cytoplasmic proteins into the nucleus does not occur during the isolation.

4. Nuclei prepared in non-aqueous media contain considerably more protein and a very different enzyme composition from that observed in nuclei prepared by "homogenization" techniques in dilute citric acid.

5. The suitability of nuclei prepared in organic media for the study of intracellular enzyme distribution is discussed.

Submitted on June 29, 1951


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 Cold Spring Harb Symp Quant BiolHome page
A. E. Mirsky, S. Osawa, and V. G. Allfrey
The Nucleus as a Site of Biochemical Activity
Cold Spring Harb Symp Quant Biol, January 1, 1956; 21(0): 49 - 73.
[Abstract] [PDF]


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