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The Journal of General Physiology, Vol 36, 1-16, Copyright © 1952 by The Rockefeller University Press

ARTICLE

SULFHYDRYL GROUPS AND THE INTERACTION BETWEEN THE HEMES IN HEMOGLOBIN

Austen F. Riggs 1

1 From the Biological Laboratories of Harvard University, Cambridge

1. Dialysis of a hemoglobin solution against slightly alkaline buffer (pH 8.68) causes a decrease in heme-heme interaction. The value of n in Hill's equation drops from 2.9 to 2.0, while the oxygen affinity increases slightly. Addition of glutathione largely reverses the effects of dialysis (n rises from 2.0 to 2.5 to 2.6).

2. Addition of the sulfhydryl reagent, p-chloromercuribenzoate (1.8 x 10–3 M), results in a large though not complete blocking of heme-heme interaction. The value of n drops from 2.9 to 1.4. This effect is largely reversed with glutathione (n rises to 2.6).

Submitted on March 6, 1952


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