¹ From the Department of Veterinary Physiology, University of Sydney, Sydney, Australia

1. Ram and bull spermatozoa suspended in a glucose-sodium chloride solution rapidly lose motility at relatively high dilutions. The substitution of chloride-free diluents does not alter the phenomenon.

2. The rapid immobilization of ram and bull spermatozoa due to high dilution may be partially prevented by the addition of supernatants of either ram or bull semen, although motility is not maintained at the same level as in a more concentrated specimen. Various other substances which also partially protect spermatozoa are egg albumin, plasma albumin, plasma gamma globulin, starch, and glycogen.

3. Washing ram spermatozoa six times greatly reduces motility. This is not restored by the addition of ram seminal plasma which, however, reverses the concurrent head agglutination.

4. Washing ram and bull spermatozoa four times results in considerable loss of motility and head agglutination both of which may be reversed by the addition of seminal plasma.

5. Potassium chloride at 0.005 M concentration partially restores the motility of four times washed ram spermatozoa at 24°C. or 37°C. but not that of similarly treated bull spermatozoa.

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