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The Journal of General Physiology, Vol 36, 631-641, Copyright © 1953 by The Rockefeller University Press

ARTICLE

ENZYME FORMATION IN NON-VIABLE CELLS

L. S. Baron 1, S. Spiegelman 1, and H. Quastler 1

1 From the Departments of Bacteriology and Physics, University of Illinois, Urbana

An attempt has been made to find conditions which would completely dissociate viability from capacity to synthesize enzymes in yeast cells. The only lethal agent or condition found which yielded complete dissociation was x-irradiation. Dosages leading to 99.9 per cent lethality and greater exhibited no inhibitory action on the capacity of the treated suspensions to synthesize maltozymase and galactozymase. Such suspensions also retained their ability to assimilate externally supplied nitrogen and employ it in the synthesis of enzyme.

Submitted on December 8, 1952


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