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The Journal of General Physiology, Vol 40, 809-825, Copyright © 1957 by The Rockefeller University Press

ARTICLE

THE PROTEIN COATS OR "GHOSTS" OF COLIPHAGE T2

I. PREPARATION, ASSAY, AND SOME CHEMICAL PROPERTIES



Roger M. Herriott 1 and James L. Barlow 1

1 From the Department of Biochemistry, The Johns Hopkins University School of Hygiene and Public Health, Baltimore

A method of preparing the protein coats or ghosts of phage T2 is described along with proof that the lytic action is a property of the ghost.

An assay based on the lytic action toward host cells has been developed which permits a rapid evaluation of the number of ghosts with a reliability of ±15 per cent.

The antigenic and certain physicochemical properties of the ghost have been determined.

Submitted on November 5, 1956


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