|
|
|
|
|
|
|
||
ARTICLE |
The uptake of gases such as oxygen, carbon monoxide, or nitric oxide by the erythrocyte involves: (a) diffusion across the cellular membrane, (b) intraerythrocytic diffusion, and (c) chemical combination with hemoglobin. The aim of this investigation was to obtain data which would permit an analysis of each of these factors in limiting the rate of gas uptake.
The initial over-all rate of uptake of gases which combine chemically with hemoglobin to produce a color change can be measured by a modified version of the Hartridge-Roughton-Millikan constant flow, rapid reaction apparatus. If nitric oxide is the reactant gas, only (a) and (b) are measured since the chemical combination of this gas with hemoglobin is extremely rapid. Our studies have shown that human biconcave discoidal erythrocytes at 38 and 48°C., have the same initial rate of carbon monoxide and nitric oxide uptake as the same cells converted into spherocytes of equal volume. Similarly there was no difference between discs and cells sphered with a 30 per cent increase in volume. Shrunken erythrocytes showed a marked decrease in rate of gas uptake. This suggests that surface area and maximum linear distance for intracellular diffusion of this magnitude do not measurably retard gas uptake. In the shrunken cells, a change in the orientation and concentration of intraerythrocytic hemoglobin and/or of the membrane components may have impeded gas diffusion.
Submitted on February 27, 1958
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  P. C. Minneci, K. J. Deans, S. Shiva, H. Zhi, S. M. Banks, S. Kern, C. Natanson, S. B. Solomon, and M. T. Gladwin Nitrite reductase activity of hemoglobin as a systemic nitric oxide generator mechanism to detoxify plasma hemoglobin produced during hemolysis Am J Physiol Heart Circ Physiol, August 1, 2008; 295(2): H743 - H754. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Sakai, A. Sato, K. Masuda, S. Takeoka, and E. Tsuchida Encapsulation of Concentrated Hemoglobin Solution in Phospholipid Vesicles Retards the Reaction with NO, but Not CO, by Intracellular Diffusion Barrier J. Biol. Chem., January 18, 2008; 283(3): 1508 - 1517. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Sonveaux, I. I. Lobysheva, O. Feron, and T. J. McMahon Transport and Peripheral Bioactivities of Nitrogen Oxides Carried by Red Blood Cell Hemoglobin: Role in Oxygen Delivery Physiology, April 1, 2007; 22(2): 97 - 112. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Liu, Q. Yan, K. L. Baskerville, and J. L. Zweier Estimation of Nitric Oxide Concentration in Blood for Different Rates of Generation: EVIDENCE THAT INTRAVASCULAR NITRIC OXIDE LEVELS ARE TOO LOW TO EXERT PHYSIOLOGICAL EFFECTS J. Biol. Chem., March 23, 2007; 282(12): 8831 - 8836. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. W. Allen and C. A. Piantadosi How do red blood cells cause hypoxic vasodilation? The SNO-hemoglobin paradigm Am J Physiol Heart Circ Physiol, October 1, 2006; 291(4): H1507 - H1512. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Borland, H. Dunningham, F. Bottrill, and A. Vuylsteke Can a membrane oxygenator be a model for lung NO and CO transfer? J Appl Physiol, May 1, 2006; 100(5): 1527 - 1538. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. B. Kim-Shapiro, A. N. Schechter, and M. T. Gladwin Unraveling the Reactions of Nitric Oxide, Nitrite, and Hemoglobin in Physiology and Therapeutics Arterioscler. Thromb. Vasc. Biol., April 1, 2006; 26(4): 697 - 705. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Chen, D. Jaron, K. A. Barbee, and D. G. Buerk The influence of radial RBC distribution, blood velocity profiles, and glycocalyx on coupled NO/O2 transport J Appl Physiol, February 1, 2006; 100(2): 482 - 492. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kavdia and A. S. Popel Venular endothelium-derived NO can affect paired arteriole: a computational model Am J Physiol Heart Circ Physiol, February 1, 2006; 290(2): H716 - H723. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Azarov, K. T. Huang, S. Basu, M. T. Gladwin, N. Hogg, and D. B. Kim-Shapiro Nitric Oxide Scavenging by Red Blood Cells as a Function of Hematocrit and Oxygenation J. Biol. Chem., November 25, 2005; 280(47): 39024 - 39032. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kavdia and A. S. Popel Contribution of nNOS- and eNOS-derived NO to microvascular smooth muscle NO exposure J Appl Physiol, July 1, 2004; 97(1): 293 - 301. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. R. Phansalkar, C. M. Hanson, A. R. Shakir, R. L. Johnson Jr., and C. C. W. Hsia Nitric Oxide Diffusing Capacity and Alveolar Microvascular Recruitment in Sarcoidosis Am. J. Respir. Crit. Care Med., May 1, 2004; 169(9): 1034 - 1040. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. M. Tsoukias, M. Kavdia, and A. S. Popel A theoretical model of nitric oxide transport in arterioles: frequency- vs. amplitude-dependent control of cGMP formation Am J Physiol Heart Circ Physiol, March 1, 2004; 286(3): H1043 - H1056. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J Pepke-Zaba and N W Morrell Pulmonary hypertension in patients with COPD: NO treatment? Thorax, April 1, 2003; 58(4): 283 - 284. [Full Text] [PDF]
|
|
|
|
|
|
M. Kavdia, N. M. Tsoukias, and A. S. Popel Model of nitric oxide diffusion in an arteriole: impact of hemoglobin-based blood substitutes Am J Physiol Heart Circ Physiol, June 1, 2002; 282(6): H2245 - H2253. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. M. Tsoukias and A. S. Popel Erythrocyte consumption of nitric oxide in presence and absence of plasma-based hemoglobin Am J Physiol Heart Circ Physiol, June 1, 2002; 282(6): H2265 - H2277. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Borland, B. Mist, M. Zammit, and A. Vuylsteke Steady-state measurement of NO and CO lung diffusing capacity on moderate exercise in men J Appl Physiol, February 1, 2001; 90(2): 538 - 544. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Philippides, P. Husbands, and M. O'Shea Four-Dimensional Neuronal Signaling by Nitric Oxide: A Computational Analysis J. Neurosci., February 1, 2000; 20(3): 1199 - 1207. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. W. Vaughn, K.-T. Huang, L. Kuo, and J. C. Liao Erythrocytes Possess an Intrinsic Barrier to Nitric Oxide Consumption J. Biol. Chem., January 28, 2000; 275(4): 2342 - 2348. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M. Kelley and A. B. DuBois Comparison between the uptake of nitrous oxide and nitric oxide in the human nose J Appl Physiol, October 1, 1998; 85(4): 1203 - 1209. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Liu, M. J. S. Miller, M. S. Joshi, H. Sadowska-Krowicka, D. A. Clark, and J. R. Lancaster Jr. Diffusion-limited Reaction of Free Nitric Oxide with Erythrocytes J. Biol. Chem., July 24, 1998; 273(30): 18709 - 18713. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Heller and K.-D. Schuster Role of reaction resistance in limiting carbon monoxide uptake in rabbit lungs J Appl Physiol, June 1, 1998; 84(6): 2066 - 2069. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Heller and K.-D. Schuster Theta values for C16O18O and C18O2 related to respective pulmonary diffusing capacities Am J Physiol Regulatory Integrative Comp Physiol, May 1, 1998; 274(5): R1496 - R1499. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. W. Vaughn, L. Kuo, and J. C. Liao Effective diffusion distance of nitric oxide in the microcirculation Am J Physiol Heart Circ Physiol, May 1, 1998; 274(5): H1705 - H1714. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. B. Dubois, J. S. Douglas, J. T. Stitt, and V. Mohsenin Production and absorption of nitric oxide gas in the nose J Appl Physiol, April 1, 1998; 84(4): 1217 - 1224. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Heller, G. Fuchs, and K.-D. Schuster Pulmonary diffusing capacities for oxygen-labeled CO2 and nitric oxide in rabbits J Appl Physiol, February 1, 1998; 84(2): 606 - 611. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kavdia, N. M. Tsoukias, and A. S. Popel Model of nitric oxide diffusion in an arteriole: impact of hemoglobin-based blood substitutes Am J Physiol Heart Circ Physiol, June 1, 2002; 282(6): H2245 - H2253. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. M. Tsoukias and A. S. Popel Erythrocyte consumption of nitric oxide in presence and absence of plasma-based hemoglobin Am J Physiol Heart Circ Physiol, June 1, 2002; 282(6): H2265 - H2277. [Abstract] [Full Text] [PDF]
|
|
|
|
