The Journal of General Physiology
Axon Instruments microelectrode amplifiers
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text (PDF, 1322K)
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JGP
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Davison, P. F.
Right arrow Articles by Taylor, E. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Davison, P. F.
Right arrow Articles by Taylor, E. W.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
The Journal of General Physiology, Vol 43, 801-823, Copyright © 1960 by The Rockefeller University Press

ARTICLE

Physical-Chemical Studies of Proteins of Squid Nerve Axoplasm, with Special Reference to the Axon Fibrous Protein

Peter F. Davison 1 and Edwin W. Taylor 1

1 From the Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts.

Dr. Taylor's present address is Committee on Biophysics, University of Chicago, Chicago, Illinois

The proteins in the axoplasm of the squid, Dosidicus gigas, have been resolved electrophoretically into a major fraction including the fibrous protein, and possibly its structural subunits, and a minor fraction including at least two proteins with low sedimentation coefficients. A partially reversible change in the structure of the fibrous protein occurs under the action of 0.4 M salt or high pH. These experiments have been interpreted to indicate that in the intact fiber one, or a few, protofibrils are arranged helically or longitudinally along the fiber axis, and linked by electrostatic bonds. On the dissociation of these bonds the separated protofibrils assume a less extended form and sediment more rapidly than the intact fibers. Some material with a lower sedimentation rate is also released on the dissociation. This fraction may comprise smaller chain fragments. The volume fraction and the approximate refractive index of the fibers have been calculated.

Submitted on July 6, 1959


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Cold Spring Harb Symp Quant BiolHome page
R. K. H. Liem, C. H. Keith, J. F. Leterrier, E. Trenkner, and M. L. Shelanski
Chemistry and Biology of Neuronal and Glial Intermediate Filaments
Cold Spring Harb Symp Quant Biol, January 1, 1982; 46(0): 341 - 350.
[Abstract] [PDF]

Home page
 ScienceHome page
R. Zackroff and R. Goldman
In vitro reassembly of squid brain intermediate filaments (neurofilaments): purification by assembly-disassembly
Science, June 6, 1980; 208(4448): 1152 - 1155.
[Abstract] [PDF]

Home page
 ScienceHome page
M. L. Shelanski, S. Albert, G. H. DeVries, and W. T. Norton
Isolation of Filaments from Brain
Science, December 17, 1971; 174(4015): 1242 - 1245.
[Abstract] [PDF]

Home page
 ScienceHome page
I. Tasaki and M. Luxoro
Intracellular Perfusion of Chilean Giant Squid Axons
Science, September 18, 1964; 145(3638): 1313 - 1315.
[Abstract] [PDF]

Home page
 ScienceHome page
F. Huneeus-Cox
Electrophoretic and Immunological Studies of Squid Axoplasm Proteins
Science, March 6, 1964; 143(3610): 1036 - 1037.
[Abstract] [PDF]


  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents