The Journal of General Physiology
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The Journal of General Physiology, Vol 43, 841-851, Copyright © 1960 by The Rockefeller University Press

ARTICLE

Growth and Division of Single Cells of Higher Plants in Vitro

Ludwig Bergmann 1

1 From The Rockefeller Institute

The cultivation of single cells of Nicotiana tabacum L. var. "Samsun" and Phaseolus vulgaris L. var. "Early Golden Cluster" on a thin agar layer in Petri dishes is described. Under these conditions about 20 per cent of the cells divided repeatedly and established tissue clones which could be isolated and maintained as growing tissue cultures. It was possible also to follow the successive divisions of isolated cells and to observe their behavior during cytogenesis under the microscope.

Submitted on July 29, 1959


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