The Journal of General Physiology
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The Journal of General Physiology, Vol 44, 889-898, Copyright © 1961 by The Rockefeller University Press


ARTICLE

Studies on the Bohr Effect of Sheep Hemoglobin

Derek G. Smyth 1, Frederick C. Battaglia 1, and Giacomo Meschia 1

1 From the Departments of Biochemistry and Physiology, Yale University School of Medicine, New Haven.

Dr. Smyth's present address is The Rockefeller Institute, New York

A simple method is described for the measurement of pH changes in hemoglobin solutions on oxygenation and reduction. Data are presented establishing the absence of a Bohr effect in p-chloromercuribenzoate [PCMB] treated hemoglobin.

The influence of a number of sulfhydryl inhibitors on the Bohr effect of a hemoglobin solution is reported and an interpretation based on steric factors in the protein is proposed.

Submitted on December 23, 1960


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