The Journal of General Physiology
Track the topics, authors and articles important to you
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow PDF (Full Text)
Right arrow Alert me when this article is cited
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JGP
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Philipson, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Philipson, L.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
The Journal of General Physiology, Vol 44, 899-910, Copyright © 1961 by The Rockefeller University Press

ARTICLE

Chromatographic Separation, and Characteristics of Nucleic Acids from HeLa Cells

Lennart Philipson 1

1 From The Rockefeller Institute

The application of the phenol-duponol method to extraction of nucleic acids from HeLa cells is described. Chromatography of the phenol extract on an esterified bovine serum albumin column with a salt gradient of sodium chloride gives separation of soluble RNA, DNA, and two different high molecular RNA fractions. Ultracentrifugation of the DNA eluted from the column gives a sedimentation coefficient (s20o,w) of 38, which agrees with ultracentrifugation data on the phenol extract. The eluted RNA appears polydisperse at low ionic strength, but at high ionic strength and after alcohol precipitation two fractions with the sedimentation coefficients of 16 and 25 to 29, respectively, were obtained.

Submitted on November 3, 1960


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 ScienceHome page
M. Hayashi, M. N. Hayashi, and S. Spiegelman
Replicating Form of a Single-Stranded DNA Virus: Isolation and Properties
Science, June 21, 1963; 140(3573): 1313 - 1316.
[Abstract] [PDF]

Home page
 Cold Spring Harb Symp Quant BiolHome page
S. Spiegelman and M. Hayashi
The Present Status of the Transfer of Genetic Information and Its Control
Cold Spring Harb Symp Quant Biol, January 1, 1963; 28(0): 161 - 181.
[Abstract] [PDF]


  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents