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The Journal of General Physiology, Vol 44, 1121-1130, Copyright © 1961 by The Rockefeller University Press

ARTICLE

In vitro Studies of the Gain and Exchange of Calcium in Frog Skeletal Muscle

Ethel Cosmos 1 and Eric J. Harris 1

1 From the Biophysics Department, University College, London.

Dr. Cosmos' present address is The Institute for Muscle Disease, Inc., New York

(1) The Ca++, Na+, and K+ contents of frog sartorius muscles were found analytically after exposure to various media including some containing labeled Ca++. (2) During storage in media with 100 to 120 mM Na+ and 1 mM Ca++ both Na+ and Ca++ are gained while K+ is lost; there is a high correlation between Na+ and Ca++ gains. (3) When Ca++ gain occurs from a solution containing labeled Ca++ there is also some exchange of the original Ca++ with the labeled Ca++. The amount exchanged is considerably less (e.g. 50 per cent) than the total amount of labeled Ca++ taken up by the tissue. (4) When the external Na+ concentration is reduced to 30 mM the amount of labeled Ca++ taken up is increased. Part of the increase is attributable to a greater net gain and part to a greater degree of exchange. (5) It is pointed out that muscles which have been loaded in vitro with labeled Ca++ will not provide a valid measure of the exchangeability of the normal Ca++ content present at the time of dissection. (6) Comparison is made between results obtained using Sr89 and Ca45 as labels for the Ca++. Little, if any, difference is perceptible.

Submitted on November 11, 1960


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M. P. Blaustein and W. J. Lederer
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Physiol Rev, July 1, 1999; 79(3): 763 - 854.
[Abstract] [Full Text] [PDF]


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