The Journal of General Physiology
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The Journal of General Physiology, Vol 44, 1229-1239, Copyright © 1961 by The Rockefeller University Press

ARTICLE

Studies on Transformations of Hemophilus influenzae

II. The molecular weight of transforming DNA by sedimentation and diffusion measurements



Sol H. Goodgal 1 and Roger M. Herriott 1

1 From the Department of Biochemistry, Johns Hopkins University School of Hygiene and Public Health, Baltimore

The sedimentation and diffusion coefficients have been determined for Hemophilus influenzae transforming activity and DNA using P32-labeled DNA. The methods employed the Spinco fixed boundary separation cell for measurements of the sedimentation coefficient and the Northrop-Anson diffusion cell to determine the diffusion coefficient. There was a very close correlation between the amount of DNA and transforming activity sedimented or diffused. The sedimentation coefficient (s20°), for both biological activity and DNA was 27 and the diffusion coefficient (D20°) 1 x 10-8 cm2/sec. The molecular weight calculated from these coefficients gave a value of 16 million. There was no difference in the sedimentation coefficients for the two unlinked markers, streptomycin and erythromycin resistance, and the diffusion coefficients for single markers or the linked markers, streptomycin and cathomycin, were the same.

Submitted on October 26, 1960


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