¹ From the Worcester Foundation for Experimental Biology, Shrewsbury

The effect of insulin administration upon D-xylose-1-C¹⁴ penetration into the diaphragm and gastrocnemius muscles of functionally nephrectomized normal, hypophysectomized, and adrenalectomized rats has been examined. It was found in all groups that after the administration of tracer amounts of D-xylose, this sugar enters the cell water of diaphragm to a greater extent than in gastrocnemius muscle, both in the presence and absence of exogenous insulin. Insulin increases the apparent intracellular distribution of D-xylose in both muscles in all three types of rats. After insulin administration, the intracellular concentration of D-xylose in diaphragm muscle was estimated to be about two times greater than D-xylose concentration in plasma; D-xylose accumulation was not observed in gastrocnemius muscle of insulin-treated rats. Intracellular accumulation of D-xylose occurs in diaphragm of insulin-treated rats at plasma concentrations of D-xylose ranging from 4 to 2200 µg/ml; however, a "saturation" phenomenon appears to be operative, since intracellular distribution declines as plasma D-xylose concentration is increased within this range. A decline in intracellular D-xylose distribution also occurs in gastrocnemius as plasma D-xylose is increased, suggesting that entry into this muscle as well does not exhibit the characteristics of a simple diffusion process. The significance of these in vivo observations is briefly discussed in relation to widely accepted assumptions concerning sugar permeability in muscle.

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