The Journal of General Physiology
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The Journal of General Physiology, Vol 5, 139-142, Copyright © 1922 by The Rockefeller University Press

ARTICLE

THE STABILITY OF BACTERIAL SUSPENSIONS

V. THE REMOVAL OF ANTIBODY FROM SENSITIZED ORGANISMS.



Paul H. De Kruif 1 and John H. Northrop 1

1 From the Laboratories of The Rockefeller Institute for Medical Research.

1. The removal of antibody from Bacillus typhosus is no more complete at pH 3 than at pH 7.

2. Approximately twelve agglutinating doses are firmly combined with the organisms. Immune body in excess of this amount is easily removable by distilled water.

3. A method of testing for the presence of immune body on the organism is described which depends on the difference in the acid agglutination of sensitized and unsensitized organisms.

4. Repeated washing in distilled water will serve to remove all the immune body from sensitized bacteria.

Submitted on September 21, 1922


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