The Journal of General Physiology
Avanti Polar Lipids
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The Journal of General Physiology, Vol 5, 223-224, Copyright © 1922 by The Rockefeller University Press

ARTICLE

THE PERMEABILITY OF LIVING CELLS TO DYES AS AFFECTED BY HYDROGEN ION CONCENTRATION

Marian Irwin 1

1 From the Laboratory of Plant Physiology, Harvard University, Cambridge.

1. An accurate quantitative method of measuring the penetration of dye into the living cell is described.

2. Cresyl blue is unable to penetrate rapidly unless the pH outside the cell is decidedly greater than that inside. The rate of penetration increases with increasing pH.

3. Around pH 9 penetration of the dye is rapid while the reverse is true of exosmosis. At low pH values (5.9) exosmosis is rapid and penetration is very slow.

Submitted on October 9, 1922


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