The Journal of General Physiology
VISIT JCB ONLINE!
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text (PDF, 849K)
Right arrow Alert me when this article is cited
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JGP
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kutchai, H.
Right arrow Articles by Staub, N. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kutchai, H.
Right arrow Articles by Staub, N. C.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
The Journal of General Physiology, Vol 53, 576-589, Copyright © 1969 by The Rockefeller University Press

ARTICLE

Steady-State, Hemoglobin-Facilitated O2 Transport in Human Erythrocytes

Howard Kutchai 1 and Norman C. Staub 1

1 From the Department of Physiology and the Cardiovascular Research Institute, University of California San Francisco Medical Center, San Francisco, California 94122.

Dr. Kutchai's present address is School of Public Health, University of Michigan, Ann Arbor, Michigan 48104

We measured the rate of oxygen transport through thin (165 µ) films of packed erythrocytes (Hb concentration = 30 g/100 ml). Under optimal conditions steady-state O2 diffusion was nearly three times that found when the hemoglobin was prevented from acting as a carrier molecule by carbon monoxide binding or high oxygen back pressure. After each experiment we measured hemolysis and found that it averaged less than 1%. Hemolysis could not account for the facilitation, thus proving that facilitated transport of O2 by hemoglobin can occur in red blood cells. The rate of facilitated transport was identical for Hb solutions of equal concentration to the cells. We interpret this to mean that under the conditions of our experiments the red cell membrane offers no detectable diffusion resistance to O2 and that the mobility of Hb in intact red cells is the same as in concentrated Hb solution.

Submitted on April 11, 1968


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
X. Liu, Q. Yan, K. L. Baskerville, and J. L. Zweier
Estimation of Nitric Oxide Concentration in Blood for Different Rates of Generation: EVIDENCE THAT INTRAVASCULAR NITRIC OXIDE LEVELS ARE TOO LOW TO EXERT PHYSIOLOGICAL EFFECTS
J. Biol. Chem., March 23, 2007; 282(12): 8831 - 8836.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. Liu, A. Samouilov, J. R. Lancaster Jr., and J. L. Zweier
Nitric Oxide Uptake by Erythrocytes Is Primarily Limited by Extracellular Diffusion Not Membrane Resistance
J. Biol. Chem., July 12, 2002; 277(29): 26194 - 26199.
[Abstract] [Full Text] [PDF]


  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents