The Journal of General Physiology
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The Journal of General Physiology, Vol 54, 123-133, Copyright © 1969 by The Rockefeller University Press

ARTICLE

Inhibition of Adenosine Triphosphatase in Sheep Red Cell Membranes by Oxidized Glutathione

D. A. T. Dick 1, E. G. Dick 1, and D. C. Tosteson 1

1 From the Department of Physiology and Pharmacology, Duke University Medical Center, Durham, North Carolina 27706.

The present address of the Drs. Dick is Department of Anatomy, The University of Dundee, Dundee, Scotland

This paper reports inhibition of Na+ + K+-stimulated, ouabain-inhibited adenosine triphosphatase (S-ATPase) in sheep red cell membranes by oxidized glutathione (GSSG). The results are consistent with the hypothesis that this inhibition depends upon the formation of a mixed disulfide between glutathione and -SH group(s) in the enzyme protein. Thus, inhibition of S-ATPase by GSSG proceeds more rapidly at alkaline than at neutral pH and is reversed by the addition of an excess of a compound containing reduced -SH groups (e.g. dithiothreitol). ATP protects S-ATPase against inhibition by GSSG and this protection depends on both the monovalent and divalent cation composition of the medium. Protection by ATP is more complete in the presence of K+ than in the presence of Na+.

Submitted on November 20, 1968


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