¹ From the Zoological Laboratory, Harvard University, Cambridge.

1. The nerve cord of the lobster (Homarus americanus Milne-Edwards) is very delicate and can be used as a living preparation for only a few hours after its removal from the animal.

2. During the first hour or so after removal it discharges CO₂ at a steadily decreasing rate beginning at about 0.20 mg. CO₂ per gram of cord per minute and ending at about 0.07 mg.

3. This discharge exhibits a steady decrease in rate and is not divisible into a period of gush and a period of uniform outflow as with the lateral-line nerve of the dogfish. It terminates in a very few hours with the complete death of the cord.

4. Both handling and cutting the cord temporarily increase the rate of CO₂ output.

5. The stimulated cord discharges CO₂ at a rate about 26 per cent higher than that of the quiescent cord, an increase of about 1.6 times that of the increase observed in the lateral-line nerve of the dogfish under similar circumstances.

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