|
|
|
|
|
|
|
||
The Journal of General Physiology, Vol 89, 379-404, Copyright © 1987 by The Rockefeller University Press
ARTICLES |
TE Decoursey, KG Chandy, S Gupta and MD Cahalan
The properties of two types of K+ channels in murine T lymphocytes are described on the basis of whole-cell and isolated-patch recordings using the gigohm-seal technique. Type l (standing for "lpr gene locus" or "large") channels were characterized mainly in T cells from mutant MRL/MpJ-lpr/lpr mice, in which they are present in large numbers. Type n ("normal") K+ channels are abundant and therefore most readily studied in concanavalin A-activated T cells from four strains of mice, MRL-+/+, CBA/J, C57BL/6J, and BALB/c. Type l channels, compared with type n, are activated at potentials approximately 30 mV more positive, and close much more rapidly upon repolarization. Type l channels inactivate more slowly and less completely than type n during maintained depolarization, but recover from inactivation more rapidly, so that little inactivation accumulates during repetitive pulses. Type l channels have a higher unitary conductance (21 pS) than type n (12 pS) and are less sensitive to block by external Co++, but are 100-fold more sensitive to block by external tetraethylammonium (TEA), with half- block of type l channels at 50-100 microM TEA compared with 8-16 mM for type n. TEA blocks both types of channels by reducing the apparent single channel current amplitude, with a dose-response relation similar to that for blocking macroscopic currents. Murine type n K+ channels resemble K+ channels in human T cells.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. Jin, B. N. Desai, B. Navarro, A. Donovan, N. C. Andrews, and D. E. Clapham Deletion of Trpm7 Disrupts Embryonic Development and Thymopoiesis Without Altering Mg2+ Homeostasis Science, October 31, 2008; 322(5902): 756 - 760. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Beeton and K. G. Chandy Potassium Channels, Memory T Cells, and Multiple Sclerosis Neuroscientist, December 1, 2005; 11(6): 550 - 562. [Abstract] [PDF]
|
|
|
|
 |
|
 H. Wulff, H.-G. Knaus, M. Pennington, and K. G. Chandy K+ Channel Expression during B Cell Differentiation: Implications for Immunomodulation and Autoimmunity J. Immunol., July 15, 2004; 173(2): 776 - 786. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. A. Koni, R. Khanna, M. C. Chang, M. D. Tang, L. K. Kaczmarek, L. C. Schlichter, and R. A. Flavell Compensatory Anion Currents in Kv1.3 Channel-deficient Thymocytes J. Biol. Chem., October 10, 2003; 278(41): 39443 - 39451. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q.-H. Liu, B. K. Fleischmann, B. Hondowicz, C. C. Maier, L. A. Turka, K. Yui, M. I. Kotlikoff, A. D. Wells, and B. D. Freedman Modulation of Kv Channel Expression and Function by TCR and Costimulatory Signals during Peripheral CD4+ Lymphocyte Differentiation J. Exp. Med., October 7, 2002; 196(7): 897 - 909. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Beeton, H. Wulff, J. Barbaria, O. Clot-Faybesse, M. Pennington, D. Bernard, M. D. Cahalan, K. G. Chandy, and E. Beraud Selective blockade of T lymphocyte K+ channels ameliorates experimental autoimmune encephalomyelitis, a model for multiple sclerosis PNAS, November 20, 2001; 98(24): 13942 - 13947. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. McCormack, K. McCormack, M. S. Nadal, E. Vieira, A. Ozaita, and B. Rudy The Effects of Shaker beta -Subunits on the Human Lymphocyte K+ Channel Kv1.3 J. Biol. Chem., July 16, 1999; 274(29): 20123 - 20126. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Fan and K. B. Walsh Mechanical Stimulation Regulates Voltage-Gated Potassium Currents in Cardiac Microvascular Endothelial Cells Circ. Res., March 5, 1999; 84(4): 451 - 457. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. H. Spencer, Y. Sokolov, H. Li, B. Takenaka, A. J. Milici, J. Aiyar, A. Nguyen, H. Park, B. K. Jap, J. E. Hall, et al. Purification, Visualization, and Biophysical Characterization of Kv1.3 Tetramers J. Biol. Chem., January 24, 1997; 272(4): 2389 - 2395. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Chandy, C. Williams, R. Spencer, B. Aguilar, S Ghanshani, B. Tempel, and G. Gutman A family of three mouse potassium channel genes with intronless coding regions Science, February 23, 1990; 247(4945): 973 - 975. [Abstract] [PDF]
|
|
|
|
|
|
R. Lewis and M. Cahalan Subset-specific expression of potassium channels in developing murine T lymphocytes Science, February 12, 1988; 239(4841): 771 - 775. [Abstract] [PDF]
|
|
|
|
