|
|
|
|
|
|
|
||
The Journal of General Physiology, Vol 96, 1247-1269, Copyright © 1990 by The Rockefeller University Press
ARTICLES |
S Liu, D Piwnica-Worms and M Lieberman
Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710.
The contribution of Cl-/HCO3- exchange to intracellular pH (pHi) regulation in cultured chick heart cells was evaluated using ion- selective microelectrodes to monitor pHi, Na+ (aiNa), and Cl- (aiCl) activity. In (HCO3- + CO2)-buffered solution steady-state pHi was 7.12. Removing (HCO3- + CO2) buffer caused a SITS (0.1 mM)-sensitive alkalinization and countergradient increase in aiCl along with a transient DIDS-sensitive countergradient decrease in aiNa. SITS had no effect on the rate of pHi recovery from alkalinization. When (HCO3- + CO2) was reintroduced the cells rapidly acidified, aiNa increased, aiCl decreased, and pHi recovered. The decrease in aiCl and the pHi recovery were SITS sensitive. Cells exposed to 10 mM NH4Cl became transiently alkaline concomitant with an increase in aiCl and a decrease in aiNa. The intracellular acidification induced by NH4Cl removal was accompanied by a decrease in aiCl and an increase in aiNa that led to the recovery of pHi. In the presence of (HCO3- + CO2), addition of either amiloride (1 mM) or DIDS (1 mM) partially reduced pHi recovery, whereas application of amiloride plus DIDS completely inhibited the pHi recovery and the decrease in aiCl. Therefore, after an acid load pHi recovery is HCO3o- and Nao- dependent and DIDS sensitive (but not Ca2+o dependent). Furthermore, SITS inhibition of Na(+)-dependent Cl-/HCO3- exchange caused an increase in aiCl and a decrease in the 36Cl efflux rate constant and pHi. In (HCO3- + CO2)-free solution, amiloride completely blocked the pHi recovery from acidification that was induced by removal of NH4Cl. Thus, both Na+/H+ and Na(+)-dependent Cl-/HCO3- exchange are involved in pHi regulation from acidification. When the cells became alkaline upon removal of (HCO3- + CO2), a SITS-sensitive increase in pHi and aiCl was accompanied by a decrease of aiNa, suggesting that the HCO3- efflux, which can attenuate initial alkalinization, is via a Na(+)-dependent Cl-/HCO3- exchange. However, the mechanism involved in pHi regulation from alkalinization is yet to be established. In conclusion, in cultured chick heart cells the Na(+)- dependent Cl-/HCO3- exchange regulates pHi response to acidification and is involved in the steady-state maintenance of pHi.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. J. Liu Inhibition of L-type Ca2+ channel current and negative inotropy induced by arachidonic acid in adult rat ventricular myocytes Am J Physiol Cell Physiol, November 1, 2007; 293(5): C1594 - C1604. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. P. Versluis, J. W. Heslinga, P. Sipkema, and N. Westerhof Contractile reserve but not tension is reduced in monocrotaline-induced right ventricular hypertrophy Am J Physiol Heart Circ Physiol, March 1, 2004; 286(3): H979 - H984. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M.J Lamers Unmasking of a novel target for blocking harmful Na+ coupled acid extrusion: electrogenic Na+-HCO3- symport Cardiovasc Res, December 1, 2001; 52(3): 339 - 344. [Full Text] [PDF]
|
|
|
|
|
|
C. M. Sciortino, L. D. Shrode, B. R. Fletcher, P. J. Harte, and M. F. Romero Localization of endogenous and recombinant Na+-driven anion exchanger protein NDAE1 from Drosophila melanogaster Am J Physiol Cell Physiol, August 1, 2001; 281(2): C449 - C463. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-M. Pei, J.-J. Zhou, J.-S. Bian, X.-C. Yu, M.-L. Fung, and T.-M. Wong Impaired [Ca2+]i and pHi responses to kappa -opioid receptor stimulation in the heart of chronically hypoxic rats Am J Physiol Cell Physiol, November 1, 2000; 279(5): C1483 - C1494. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. Hume, D. Duan, M. L. Collier, J. Yamazaki, and B. Horowitz Anion Transport in Heart Physiol Rev, January 1, 2000; 80(1): 31 - 81. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. Melvin, K. Park, L. Richardson, P. J. Schultheis, and G. E. Shull Mouse Down-regulated in Adenoma (DRA) Is an Intestinal Cl-/HCO3- Exchanger and Is Up-regulated in Colon of Mice Lacking the NHE3 Na+/H+ Exchanger J. Biol. Chem., August 6, 1999; 274(32): 22855 - 22861. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Carmeliet Cardiac Ionic Currents and Acute Ischemia: From Channels to Arrhythmias Physiol Rev, July 1, 1999; 79(3): 917 - 1017. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Sun, R. D. Vaughan-Jones, and J.-I. Kambayashi Two distinct HCO-3-dependent H+ efflux pathways in human vascular endothelial cells Am J Physiol Heart Circ Physiol, July 1, 1999; 277(1): H28 - H32. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Petrecca, R. Atanasiu, S. Grinstein, J. Orlowski, and A. Shrier Subcellular localization of the Na+/H+ exchanger NHE1 in rat myocardium Am J Physiol Heart Circ Physiol, February 1, 1999; 276(2): H709 - H717. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Gambassi, H. A. Spurgeon, B. D. Ziman, E. G. Lakatta, and M. C. Capogrossi Opposing effects of alpha 1-adrenergic receptor subtypes on Ca2+ and pH homeostasis in rat cardiac myocytes Am J Physiol Heart Circ Physiol, April 1, 1998; 274(4): H1152 - H1162. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Watanabe and M. Endoh Relationship between the increase in Ca2+ transient and contractile force induced by angiotensin II in aequorin-loaded rabbit ventricular myocardium Cardiovasc Res, February 1, 1998; 37(2): 524 - 531. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Yamaguchi, M. Tamagawa, N. Nakajima, and Haruaki Nakaya Selective impairment of HCO3--dependent pHi regulation by lysophosphatidylcholine in guinea pig ventricular myocardium Cardiovasc Res, January 1, 1998; 37(1): 179 - 186. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Le Prigent, D. Lagadic-Gossmann, E. Mongodin, and D. Feuvray HCO−3-dependent alkalinizing transporter in adult rat ventricular myocytes: characterization and modulation Am J Physiol Heart Circ Physiol, December 1, 1997; 273(6): H2596 - H2603. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. C. Camilion de Hurtado, B. V. Alvarez, N. G. Perez, and H. E. Cingolani Role of an Electrogenic Na+-HCO3- Cotransport in Determining Myocardial pHi After an Increase in Heart Rate Circ. Res., October 1, 1996; 79(4): 698 - 704. [Abstract] [Full Text]
|
|
|
|
 |
|
 T. Ohashi, F. Yamamoto, H. Yamamoto, H. Ichikawa, T. Shibata, and Y. Kawashima TRANSIENT REPERFUSION WITH ACIDIC SOLUTION AFFECTS POSTISCHEMIC FUNCTIONAL RECOVERY: STUDIES IN THE ISOLATED WORKING RAT HEART J. Thorac. Cardiovasc. Surg., March 1, 1996; 111(3): 613 - 620. [Abstract] [Full Text]
|
|
|
|
|
|
I. Korichneva, M. Puceat, R. Cassoly, and G. Vassort Cl--HCO3- Exchange in Developing Neonatal Rat Cardiac Cells : Biochemical Identification and Immunolocalization of Band 3–Like Proteins Circ. Res., September 1, 1995; 77(3): 556 - 564. [Abstract] [Full Text]
|
|
|
|
|
|
M. Pucéat, I. Korichneva, R. Cassoly, and G. Vassort Identification of Band 3-like Proteins and Cl[IMAGE]/HCO(3)[IMAGE] Exchange in Isolated Cardiomyocytes J. Biol. Chem., January 20, 1995; 270(3): 1315 - 1322. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Ziegelstein, L Cheng, and M. Capogrossi Flow-dependent cytosolic acidification of vascular endothelial cells Science, October 23, 1992; 258(5082): 656 - 659. [Abstract] [PDF]
|
|
|
|
